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A worldwide multicentre comparison of assays for cerebrospinal fluid biomarkers in Alzheimer's disease.

Verwey, N A (author)
van der Flier, W M (author)
Blennow, Kaj, 1958 (author)
Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
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Clark, C (author)
Sokolow, S (author)
De Deyn, P P (author)
Galasko, D (author)
Hampel, H (author)
Hartmann, T (author)
Kapaki, E (author)
Lannfelt, Lars (author)
Uppsala universitet,Geriatrik
Mehta, P D (author)
Parnetti, L (author)
Petzold, A (author)
Pirttila, T (author)
Saleh, L (author)
Skinningsrud, A (author)
Swieten, J C V (author)
Verbeek, M M (author)
Wiltfang, J (author)
Younkin, S (author)
Scheltens, P (author)
Blankenstein, M A (author)
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 (creator_code:org_t)
2009-04-02
2009
English.
In: Annals of clinical biochemistry. - : SAGE Publications. - 0004-5632 .- 1758-1001. ; 46:Pt 3, s. 235-40
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • BACKGROUND: Different cerebrospinal fluid (CSF) amyloid-beta 1-42 (Abeta(1-42)), total Tau (Tau) and Tau phosphorylated at threonine 181 (P-Tau) levels are reported, but currently there is a lack of quality control programmes. The aim of this study was to compare the measurements of these CSF biomarkers, between and within centres. METHODS: Three CSF-pool samples were distributed to 13 laboratories in 2004 and the same samples were again distributed to 18 laboratories in 2008. In 2004 six laboratories measured Abeta(1-42), Tau and P-Tau and seven laboratories measured one or two of these marker(s) by enzyme-linked immunosorbent assays (ELISAs). In 2008, 12 laboratories measured all three markers, three laboratories measured one or two marker(s) by ELISAs and three laboratories measured the markers by Luminex. RESULTS: In 2004, the ELISA intercentre coefficients of variance (interCV) were 31%, 21% and 13% for Abeta(1-42), Tau and P-Tau, respectively. These were 37%, 16% and 15%, respectively, in 2008. When we restricted the analysis to the Innotest (N = 13) for Abeta(1-42), lower interCV were calculated (22%). The centres that participated in both years (N = 9) showed interCVs of 21%, 15% and 9% and intra-centre coefficients (intraCV) of variance of 25%,18% and 7% in 2008. CONCLUSIONS: The highest variability was found for Abeta(1-42). The variabilities for Tau and P-Tau were lower in both years. The centres that participated in both years showed a high intraCV comparable to their interCV, indicating that there is not only a high variation between but also within centres. Besides a uniform standardization of (pre)analytical procedures, the same assay should be used to decrease the inter/intracentre variation.

Keyword

Alzheimer Disease
cerebrospinal fluid
Amyloid beta-Protein
cerebrospinal fluid
Biological Assay
methods
Enzyme-Linked Immunosorbent Assay
methods
Humans
Reproducibility of Results
tau Proteins
cerebrospinal fluid
MEDICINE

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ref (subject category)
art (subject category)

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