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A worldwide multice...
A worldwide multicentre comparison of assays for cerebrospinal fluid biomarkers in Alzheimer's disease.
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Verwey, N A (author)
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van der Flier, W M (author)
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- Blennow, Kaj, 1958 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
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Clark, C (author)
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Sokolow, S (author)
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De Deyn, P P (author)
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Galasko, D (author)
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Hampel, H (author)
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Hartmann, T (author)
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Kapaki, E (author)
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- Lannfelt, Lars (author)
- Uppsala universitet,Geriatrik
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Mehta, P D (author)
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Parnetti, L (author)
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Petzold, A (author)
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Pirttila, T (author)
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Saleh, L (author)
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Skinningsrud, A (author)
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Swieten, J C V (author)
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Verbeek, M M (author)
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Wiltfang, J (author)
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Younkin, S (author)
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Scheltens, P (author)
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Blankenstein, M A (author)
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(creator_code:org_t)
- 2009-04-02
- 2009
- English.
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In: Annals of clinical biochemistry. - : SAGE Publications. - 0004-5632 .- 1758-1001. ; 46:Pt 3, s. 235-40
- Related links:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
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- BACKGROUND: Different cerebrospinal fluid (CSF) amyloid-beta 1-42 (Abeta(1-42)), total Tau (Tau) and Tau phosphorylated at threonine 181 (P-Tau) levels are reported, but currently there is a lack of quality control programmes. The aim of this study was to compare the measurements of these CSF biomarkers, between and within centres. METHODS: Three CSF-pool samples were distributed to 13 laboratories in 2004 and the same samples were again distributed to 18 laboratories in 2008. In 2004 six laboratories measured Abeta(1-42), Tau and P-Tau and seven laboratories measured one or two of these marker(s) by enzyme-linked immunosorbent assays (ELISAs). In 2008, 12 laboratories measured all three markers, three laboratories measured one or two marker(s) by ELISAs and three laboratories measured the markers by Luminex. RESULTS: In 2004, the ELISA intercentre coefficients of variance (interCV) were 31%, 21% and 13% for Abeta(1-42), Tau and P-Tau, respectively. These were 37%, 16% and 15%, respectively, in 2008. When we restricted the analysis to the Innotest (N = 13) for Abeta(1-42), lower interCV were calculated (22%). The centres that participated in both years (N = 9) showed interCVs of 21%, 15% and 9% and intra-centre coefficients (intraCV) of variance of 25%,18% and 7% in 2008. CONCLUSIONS: The highest variability was found for Abeta(1-42). The variabilities for Tau and P-Tau were lower in both years. The centres that participated in both years showed a high intraCV comparable to their interCV, indicating that there is not only a high variation between but also within centres. Besides a uniform standardization of (pre)analytical procedures, the same assay should be used to decrease the inter/intracentre variation.
Keyword
- Alzheimer Disease
- cerebrospinal fluid
- Amyloid beta-Protein
- cerebrospinal fluid
- Biological Assay
- methods
- Enzyme-Linked Immunosorbent Assay
- methods
- Humans
- Reproducibility of Results
- tau Proteins
- cerebrospinal fluid
- MEDICINE
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Verwey, N A
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van der Flier, W ...
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Blennow, Kaj, 19 ...
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Clark, C
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Sokolow, S
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De Deyn, P P
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show more...
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Galasko, D
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Hampel, H
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Hartmann, T
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Kapaki, E
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Lannfelt, Lars
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Mehta, P D
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Parnetti, L
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Petzold, A
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Pirttila, T
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Saleh, L
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Skinningsrud, A
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Swieten, J C V
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Verbeek, M M
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Wiltfang, J
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Younkin, S
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Scheltens, P
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Blankenstein, M ...
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show less...
- Articles in the publication
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Annals of clinic ...
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Annals of Clinic ...
- By the university
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University of Gothenburg
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Uppsala University